Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

December 15, 2023

哺乳动物细胞原位冷冻断层扫描的样品制备

准备用于细胞冷冻断层扫描的电子显微镜（EM）网格

用于冷冻断层扫描的电子显微镜（EM）网格上的细胞接种和转染

用于冷冻断层扫描的电子显微镜（EM）网格的浸入冷冻

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Summary

该方法为原位细胞冷冻断层扫描和相关光电子显微镜（CLEM）的电子显微镜（EM）网格的制备提供了一种可访问且灵活的协议。

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