Isolating Small Extracellular Vesicles and Extracting Their Peptidome

June 30, 2023

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

Isolating Small Extracellular Vesicles (SEVs) by Differential Ultracentrifugation

Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

Jiale Cheng², Jiong Zhu², Yujiao Liu³, Chen Yang³, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Jian Wang²

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Summary

This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.

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