Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Yilun Sun

doi:10.3791/65315

Journal

Biochimie

Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks

April 21, 2023

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

03:20 min

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1,132 Views

Cell Culture and Drug Treatment in the Human Embryonic Kidney 293 (HEK293) Cell Line

01:15 min

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412 Views

Isolation and Normalization of DNA Containing Crosslinked Proteins

02:49 min

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240 Views

Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs)

03:14 min

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241 Views

Yilun Sun¹

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Summary

The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.