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June 02, 2023

荧光染色脂滴分析与3D重建，用于肝脂肪变性评估

脂滴的冷冻肝组织切片和BODIPY染色

脂滴的图像采集和分析

Karina Garcia^2,3,4, André Alves^2,3, Teresa M. Ribeiro-Rodrigues^2,3, Flávio Reis^2,3, Sofia Viana^2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Summary

在本文中，我们展示了一种优化的基于BODIPY 493 / 503荧光的方案，用于肝组织中的脂滴表征。通过使用正交投影和3D重建，荧光团可以成功区分微泡和大泡脂肪变性，并且可能代表用于肝脂肪变性评估的经典组织学方案的补充方法。

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