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Simplified and Efficient AAV Production Using HEK293 Suspension Culture
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Author Spotlight: Advancing Gene Therapy with High-Yield AAV Vectors Through HEK293 Suspension Cell Cultivation
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JoVE Revista Neurociencias
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells

Author Spotlight: Advancing Gene Therapy with High-Yield AAV Vectors Through HEK293 Suspension Cell Cultivation

Simplified and Efficient AAV Production Using HEK293 Suspension Culture

DOI:
10.3791/66532-v

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01:22 min

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April 26, 2024

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, , , , , ,
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Tags

Adeno-associated VirusAAVViral VectorHEK293 CellsSuspension CultureGene TherapyCentral Nervous SystemNeurodegenerative DiseasesMultiple SclerosisParkinson’s DiseaseAlzheimer’s DiseaseTransfectionPolyethylenimineTransItUltracentrifugationGenome Copies

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