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Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
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Echtzeit-Bildgebung der akrosomalen Kalziumdynamik und Exozytose in lebenden Mäusespermien
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JoVE Revista Biología del desarrollo
Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm
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Echtzeit-Bildgebung der akrosomalen Kalziumdynamik und Exozytose in lebenden Mäusespermien

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00:46 min

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October 13, 2023

DOI:

10.3791/65962-v

DOI:
10.3791/65962-v

•

00:46 min
October 13, 2023

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2 Views
, , , , ,
1Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, 2Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Tags

Real-time ImagingAcrosomal Calcium DynamicsSperm ExocytosisCalcium SignalingCapacitationFertilizationMembrane LipidsAcrosome-targeted Sensor For Exocytosis (AcroSensE)Genetically Encoded Calcium Indicator (GCaMP)High Frame-rate ImagingSperm ActivationMembrane FusionCRISPR Gene-editingTransgenic Mouse Model

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Cohen, R., Sosnicki, D. M., White, M. A., Nelson, J. L., Mukai, C., Travis, A. J. Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm. J. Vis. Exp. (200), e65962, doi:10.3791/65962 (2023).

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