/

/

Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

/

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

JoVE Revista
Bioquímica

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Bioquímica

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

DOI:

10.3791/65718-v

•

01:09 min

•

January 12, 2024

•

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Tags

Biophysical Assays Ternary Complex Formation Proteolysis Targeting Chimeras (PROTACs)Surface Plasmon Resonance (SPR)Biolayer Interferometry (BLI)Isothermal Titration Calorimetry (ITC)Brd4BD2 VHL PPM1D Cereblon (CRBN)Kinetics Thermodynamics Cooperativity Hook Effect

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Read Article