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High-Throughput Screening for Protein Crystallization

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Høy gjennomstrømningsscreening for å oppnå krystalltreff for proteinkrystallografi

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Bioquímica

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JoVE Revista Bioquímica

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Høy gjennomstrømningsscreening for å oppnå krystalltreff for proteinkrystallografi

High-Throughput Screening for Protein Crystallization

DOI:

10.3791/65211-v

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01:47 min

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March 10, 2023

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Gabrielle R. Budziszewski, M. Elizabeth Snell, Tiffany R. Wright, Miranda L. Lynch, Sarah E. J. Bowman²

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

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Keywords: High-throughput Screening Protein Crystallography Crystal Hits Structural Biology Computational Structure Prediction Micro ED Serial Crystallography Advanced Imaging UVTBF SHG Marco Algorithm Crystallization Cocktail Non-linear Optical Methods X-ray Crystallography 1536-well Microbatch-under-oil Plate Automated High-throughput State-of-the-art Imaging

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