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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
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בדיקות שאינן תלויות בחמצן למדידת תפקוד המיטוכונדריה ביונקים
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Bioquímica
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JoVE Revista Bioquímica
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
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בדיקות שאינן תלויות בחמצן למדידת תפקוד המיטוכונדריה ביונקים

1,287 Views

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01:14 min

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May 19, 2023

DOI:

10.3791/65184-v

DOI:
10.3791/65184-v

•

01:14 min
May 19, 2023

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15 Views
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1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

Tags

Mitochondrial FunctionOxygen-independent AssaysHypoxiaMetabolite AnalysisFumarateElectron Transport ChainATP ProductionPyrimidine BiosynthesisNADH OxidationSuperoxide ProductionRespirometry

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Tseyang, T., Valeros, J., Vo, P., Spinelli, J. B. Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals. J. Vis. Exp. (195), e65184, doi:10.3791/65184 (2023).

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