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10:01 min
December 16, 2022
DOI:
10.3791/64526-v
钙内流是T细胞信号传导的量度,是分析对T细胞受体刺激反应的有效方法。该协议将Indo-1与针对细胞表面分子的抗体组合多重化,利用了全光谱流式细胞术的高度灵活性。
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Perrenoud, L., Conley, J., Berg, L. J. Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry. J. Vis. Exp. (190), e64526, doi:10.3791/64526 (2022).
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