Quantifying the contact area and force applied by an atomic force microscope (AFM) probe tip to a sample surface enables nanoscale mechanical property determination. Best practices to implement AFM cantilever-based nanoindentation in air or fluid on soft and hard samples to measure elastic modulus or other nanomechanical properties are discussed.
Enrriques, A. E., Howard, S., Timsina, R., Khadka, N. K., Hoover, A. N., Ray, A. E., Ding, L., Onwumelu, C., Nordeng, S., Mainali, L., Uzer, G., Davis, P. H. Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid. J. Vis. Exp. (190), e64497, doi:10.3791/64497 (2022).