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Medicamenteuze behandeling door centrale veneuze katheter in een muismodel van angiotensine II geïnduceerd abdominaal aorta-aneurysma en monitoring door 3D-echografie
JoVE Revista
Medicina
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JoVE Revista Medicina
Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound
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Medicamenteuze behandeling door centrale veneuze katheter in een muismodel van angiotensine II geïnduceerd abdominaal aorta-aneurysma en monitoring door 3D-echografie

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10:09 min

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August 04, 2022

DOI:

10.3791/64124-v

DOI:
10.3791/64124-v

•

10:09 min
August 04, 2022

•

5 Views
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1Division of Vascular Surgery, Department of General Surgery,Medical University of Vienna and Vienna General Hospital, 2Leeds Institute for Cardiovascular and Metabolic Medicine, School of Medicine,University of Leeds, 3Leeds Vascular Institute,Leeds General Infirmary, 4Department for Visceral, Thoracic and Vascular Surgery,Technical University of Dresden and University Hospital Carl-Gustav Carus, 5Division of Cardiovascular and Interventional Radiology, Division of Molecular and Gender Imaging, Department of Biomedical Imaging and Image Guided Therapy,Medical University of Vienna and Vienna General Hospital

Summary

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Dit protocol beschrijft de opeenvolgende implantatie van een osmotische pomp om abdominaal aorta-aneurysma te induceren door angiotensine II-afgifte in apolipoproteïne E (ApoE) deficiënte muizen en van een vasculaire toegangspoort met een halsaderkatheter voor herhaalde medicamenteuze behandeling. Monitoring van de ontwikkeling van aneurysma's door middel van 3D-echografie wordt effectief uitgevoerd ondanks dorsale implantaten.

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Mouse ModelAbdominal Aortic AneurysmDrug TreatmentCentral Venous Catheter3D UltrasoundJugular Vein CatheterizationAPOE DeficientAnesthesiaSurgical ProcedureVascular Access SystemCatheter PreparationDisinfectionBlunt DissectionExternal Jugular Vein

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Ibrahim, N., Klopf, J., Bleichert, S., Bailey, M. A., Busch, A., Stiglbauer-Tscholakoff, A., Eilenberg, W., Neumayer, C., Brostjan, C. Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound. J. Vis. Exp. (186), e64124, doi:10.3791/64124 (2022).

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