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Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum
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Biología
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JoVE Revista Biología
Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

DOI:
10.3791/63682-v

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09:07 min

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April 06, 2022

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, ,
1Dept. Cell and Developmental Biology,Vanderbilt University, 2Program in Developmental Biology,Vanderbilt University

Capítulos

  • 00:04Introduction
  • 00:54Pupae Immobilization
  • 03:07Dissecting the Notum
  • 05:59Mounting the Notum
  • 07:47Results: Imaging of Notum
  • 08:27Conclusion

Summary

Traducción Automática

Traducción Automática

The present protocol details the preparation and visualization of fixed tissue of the Drosophila pupal notum. It can be used for either intact or wounded tissue, and the original architecture of the tissue is preserved. The procedures for dissecting, fixing, and staining are all described in this article.

Tags

DrosophilaPupaNotumImmunohistochemistryDissectionLive-imagingAntibody StainingDNA StainingIn Situ HybridizationMicroscopyForcepsAdhesive GluePBSPipette

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