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Rutinemessig innsamling av kryo-EM-datasett med høy oppløsning ved bruk av 200 KV-transmisjonselektronmikroskop
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Bioquímica
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JoVE Revista Bioquímica
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Rutinemessig innsamling av kryo-EM-datasett med høy oppløsning ved bruk av 200 KV-transmisjonselektronmikroskop

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09:49 min

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March 16, 2022

DOI:

10.3791/63519-v

DOI:
10.3791/63519-v

•

09:49 min
March 16, 2022

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6 Views
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1Materials and Structural Analysis Division,Thermo Fisher Scientific, 2Max Planck Institute of Biochemistry, Molecular Structural Biology, 3Materials and Structural Analysis Division,Thermo Fisher Scientific

Tags

Cryo-EM200 KV TEMHigh-resolutionProtein StructureProtein ComplexesMolecular MechanismsStructure-based Drug DesignApoferritinProteasomeAuto GridsLiquid NitrogenAutomated Data CollectionAtlasEPU

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Koh, A., Khavnekar, S., Yang, W., Karia, D., Cats, D., van der Ploeg, R., Grollios, F., Raschdorf, O., Kotecha, A., Němeček, D. Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope. J. Vis. Exp. (181), e63519, doi:10.3791/63519 (2022).

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