Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Characterization of Cyanobacterial…
JoVE Journal
Biology

Esto contenido es Free Access.

JoVE Journal Biology
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
 

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Article DOI: 10.3791/63481-v • 08:44 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 3,4,5, 3,4, 3,4,6, 3,4,6
1Department of Biological Sciences, Wellesley College, 2Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario, Universidad de Córdoba, 3i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 5MCbiology Doctoral Program, ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 6Departamento de Biologia, Faculdade de Ciências, Universidade do Porto
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

The present protocol providesdetailed descriptions for isolation, concentration,and characterization of extracellular vesicles from cyanobacterial cultures. Approaches for purifying vesicles from cultures at different scales, trade-offs among methodologies, and considerations for working with field samples are also discussed.

Tags

Isolation Characterization Cyanobacterial Extracellular Vesicles Purification Culture Trade Offs Methodologies Field Samples Sampling Analysis Concentration Ultrafiltration Centrifugal Concentrators Type 1 Ultrapure Grade Water Supernatant Sample Final Volume Tangential Flow Filtration Apparatus Peristaltic Pump Retentate Line Sanitize Back Pressure Levels
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter