Journal
/
Biología del desarrollo
/
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

DOI:
10.3791/63431-v

•

09:48 min

•

February 25, 2022

•

, ,
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven)

Capítulos

  • 00:04Introduction
  • 00:53Isolation of Mouse Anterior Lobe
  • 01:57Dissociation of Pituitary into Single Cells
  • 04:28Establishment and Culturing of Anterior Lobe Derived Organoids
  • 07:31Results: Establishment and Validation of Anterior Lobe Derived Organoids
  • 09:21Conclusion

Summary

Traducción Automática

Traducción Automática

The pituitary gland is the key regulator of the body's endocrine system. This article describes the development of organoids from the mouse pituitary as a novel 3D in vitro model to study the gland's stem cell population of which the biology and function remain poorly understood.

Tags

OrganoidsMouse PituitaryIn Vitro ModelPituitary Stem Cell BiologyPituitary RemodelingNeonatal MaturationAgingTumor GrowthCell IsolationTrypsinDNAseCell CultureMedium

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

Read Article