Journal
/
Neurociencias
/
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

1,970 Views

•

07:40 min

•

February 11, 2022

DOI:

10.3791/63327-v

DOI:
10.3791/63327-v

•

07:40 min
February 11, 2022

•

7 Views
, ,
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science,Aalto University School of Science, 3HiLIFE – Neuroscience Center,University of Helsinki, 4HiLIFE – Institute of Biotechnology,University of Helsinki

Summary

Automatically generated

The present protocol describes a method to visualize and measure actin rings and other components of the membrane periodic skeleton of the axon initial segment using cultured rat hippocampal neurons and 3D-structured illumination microscopy (3D-SIM).

Explore More Videos

3D-SIMMembrane Periodic SkeletonAxon Initial SegmentFluorescence MicroscopySample PreparationAnkyrin GFluoro 4FerodinMountingImmersion Oil

Read Article

Cite this Article

Micinski, D., Lahti, L., Abouelezz, A. Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM). J. Vis. Exp. (180), e63327, doi:10.3791/63327 (2022).

Download .ris file

Copy

Share Video

.

Copy