Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
人诱导多能干细胞及其神经和神经胶质衍生物中多种线粒体参数的流式细胞术分析
3,531 Views
•
06:09 min
•
November 08, 2021
DOI:
1Department of Clinical Medicine (K1),University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases,Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine,Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
Summary
Automatically generated
本研究报道了一种基于流式细胞术和两种荧光报告基因或抗体双重染色测量多种线粒体功能参数的新方法，以检测线粒体体积、线粒体膜电位、活性氧水平、线粒体呼吸链组成和线粒体 DNA 的变化。
Read Article
Cite this Article
Liang, K. X., Chen, A., Kristiansen, C. K., Bindoff, L. A. Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives. J. Vis. Exp. (177), e63116, doi:10.3791/63116 (2021).
Copy