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量化草甘膦产品对微生物组的潜在影响

JoVE Revista
Medio ambiente

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JoVE Revista Medio ambiente

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

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量化草甘膦产品对微生物组的潜在影响

DOI:

10.3791/63109-v

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07:42 min

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January 10, 2022

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Suni Anie Mathew, Anne Muola, Kari Saikkonen, Irma Saloniemi, Marjo Helander, Pere Puigbò^3,4

¹Biodiversity Unit,University of Turku, ²Department of Biology,University of Turku, ³Nutrition and Health Unit,Eurecat Technology Centre of Catalonia, ⁴Department of Biochemistry and Biotechnology,Rovira i Virgili University

Capítulos

00:05Introduction
00:52Two Field Experiments to Test the Effect of Glyphosate Based Products (GBPs)
01:58Microbiome Analyses: 16S rRNA and ITS Gene Amplification, and Library Preparation
04:59Algorithm to Determine the Potential Sensitivity of Organisms to Glyphosate
05:56Assessing the EPSPS Class from Universal Microbial Markers
06:40Results: Quantification of Microbial Species Sensitivity after GBPs Usage
07:10Conclusion

Summary

Traducción Automática

草甘膦产品（GBP）是全球最常见的广谱除草剂。在本文中，我们介绍了量化GBP对微生物组影响的一般指南，从现场实验到生物信息学分析。

Tags

Keywords: Glyphosate Microbiome 16S RRNA Experimental Design Plant Samples Quail Bedding PCR Amplification DNA Extraction Bioinformatics Workflow

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