Journal
/
Neurociencias
/
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

DOI:
10.3791/62977-v

•

11:08 min

•

December 07, 2021

•

, ,
1Zilkha Neurogenetic Institute, Keck School of Medicine,University of Southern California, 2Department of Cell & Neurobiology, Keck School of Medicine,University of Southern California

Capítulos

  • 00:04Introduction
  • 00:52Live Cell Retinal Peeling Method
  • 03:35Lyophilized Retina Peeling Method
  • 07:42Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
  • 10:23Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol presents two techniques to isolate the subcellular compartments of murine rod photoreceptors for protein analysis. The first method utilizes live retinae and cellulose filter paper to separate rod outer segments, while the second employs lyophilized retinae and adhesive tape to peel away rod inner and outer segment layers.

Tags

Photoreceptor Cell CompartmentsMouse RetinaProtein AnalysisPeeling TechniquesRod Subcellular CompartmentsRod Outer Segment (ROS)Ames HEPES BufferFilter PaperC57 Black 6J MouseRetinal DissectionProtein Analysis

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

In vivo Electroporation of Developing Mouse Retina

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Read Article