/
/
From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Introduction
- 00:36Media Growth
- 01:35Cell Harvest
- 02:34Extract Preparation
- 03:43Cell-Free Protein Synthesis and Quantification of the Reporter Protein, Super Folder Green Fluorescent Protein (sfGFP)
- 04:46Results: Comparison Between Cell Extracts Obtained from Cell-Free Autoinduction Media Grown to Optical Densities of 2.5 and 10
- 05:27Conclusion
This work describes the preparation of cell extract from Escherichia coli (E. coli) followed by cell-free protein synthesis (CFPS) reactions in under 24 hours. Explanation of the cell-free autoinduction (CFAI) protocol details improvements made to reduce researcher oversight and increase quantities of cell extract obtained.
