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क्रायो-ईएम के लिए उच्च तापमान नमूना ग्रिड की तैयारी

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Preparation of High-Temperature Sample Grids for Cryo-EM

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क्रायो-ईएम के लिए उच्च तापमान नमूना ग्रिड की तैयारी

3,200 Views

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05:05 min

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July 26, 2021

DOI:

10.3791/62772-v

DOI:

10.3791/62772-v

•

05:05 min

July 26, 2021

•

1 Views

Yuan-Chih Chang², Chin-Yu Chen⁴, Ming-Daw Tsai⁵

¹Institute of Biological Chemistry,Academia Sinica, ²Academia Sinica Cryo-EM Facility,Academia Sinica, ³Department of Life Sciences,National Central University, ⁴Hubei University, ⁵Institute of Biochemical Sciences,National Taiwan University

Tags

Cryo-EM High-temperature Sample Protein Structure Temperature Dependence Vitrification Grid Preparation Sample Application Blotting Liquid Ethane Electron Microscopy

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