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Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
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Biología
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JoVE Revista Biología
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

4,317 Views

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07:28 min

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June 01, 2021

DOI:

10.3791/62559-v

DOI:
10.3791/62559-v

•

07:28 min
June 01, 2021

•

13 Views
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1Department of Biochemistry and Molecular Biology,Colorado State University, 2World Research Hub Initiative, Institute of Innovative Research,Tokyo Institute of Technology

Summary

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Bead loading introduces proteins, plasmids, and particles into adherent mammalian cells. This cell loading technique is inexpensive, rapid, and does not substantially affect cell health. It is best suited for live-cell imaging.

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Cite this Article

Cialek, C. A., Galindo, G., Koch, A. L., Saxton, M. N., Stasevich, T. J. Bead Loading Proteins and Nucleic Acids into Adherent Human Cells. J. Vis. Exp. (172), e62559, doi:10.3791/62559 (2021).

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