Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Analysis of SAMHD1 Restriction by Flow Cytometry…
JoVE Journal
Immunology and Infection

Esto contenido es Open Access.

JoVE Journal Immunology and Infection
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
 

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

Capítulos

Resumen
Automatic Translation

Described here is an established method to determine the extent of HIV-1 restriction by the cellular inhibitory protein SAMHD1. Human myeloid lineage U937 cells are transduced with a SAMHD1 expression vector co-expressing YFP, differentiated and then challenged with HIV-RFP. The level of restriction is determined by flow cytometry analysis.

Tags

SAMHD1 Restriction Flow Cytometry Human Myeloid U937 Cells Analysis Proteins Cells Target Viruses Multicolor Flow Cytometry Cytometer Computer Analysis Software Arm Movement Water Tube Flow Rate Prime Light Indicator Specimen Blank Experiment Blank Panel Cytometer Settings Inspector Parameters Menu
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter