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使用肠道器官模型研究猿特异性相互作用的文化方法

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

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使用肠道器官模型研究猿特异性相互作用的文化方法

9,688 Views

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07:49 min

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March 23, 2021

DOI:

10.3791/62330-v

DOI:

10.3791/62330-v

•

07:49 min

March 23, 2021

•

22 Views

Georgios Stroulios*¹, Martin Stahl*², Fisal Elstone*², Wing Chang¹, Sharon Louis², Allen Eaves^2,3, Salvatore Simmini¹, Ryan K. Conder²

¹STEMCELL Technologies Ltd., ²STEMCELL Technologies Inc., ³Terry Fox Laboratory, BC Cancer

Summary

在这里，我们提出了两个协议，允许肠道特定的相互作用建模。器官衍生肠道单层和空气-液体接口（ALI）培养物有助于产生从亮面和玄武岩两侧获得分化良好的表皮，而极性倒置肠道器官暴露其腹腔侧，并适合高通量检测。

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