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Hochdurchsatz-Transkriptomanalyse zur Untersuchung von Wirt-Pathogen-Interaktionen
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JoVE Revista Inmunología y contagio
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Hochdurchsatz-Transkriptomanalyse zur Untersuchung von Wirt-Pathogen-Interaktionen

3,860 Views

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14:58 min

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March 05, 2022

DOI:

10.3791/62324-v

DOI:
10.3791/62324-v

•

14:58 min
March 05, 2022

•

3 Views
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1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Summary

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Das hier vorgestellte Protokoll beschreibt eine komplette Pipeline zur Analyse von RNA-Sequenzierungs-Transkriptomdaten von Rohlesungen bis hin zur Funktionsanalyse, einschließlich Qualitätskontroll- und Vorverarbeitungsschritten bis hin zu fortschrittlichen statistischen Analyseansätzen.

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Cite this Article

Aquime Gonçalves, A. N., Escolano Maso, V., Maia Santos de Castro, Í., Pereira Vasconcelos, A., Tomio Ogava, R. L., I Nakaya, H. High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions. J. Vis. Exp. (181), e62324, doi:10.3791/62324 (2022).

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