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Optimization of Crystal Growth for Neutron Macromolecular Crystallography
 

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Article DOI: 10.3791/61685-v • 12:29 min • March 13th, 2021
March 13th, 2021 •
1, 1,2, 1
1CEA, CNRS, IBS, Université Grenoble Alpes, 2ELVESYS SAS

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Structural studies of biomacromolecules by crystallography require high-quality crystals. Here we demonstrate a protocol that can be used by OptiCrys (a fully automated instrument developed in our lab) and/or microdialysis buttons for growing large high-quality crystals based on knowledge of the crystallization phase diagram.

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Crystal Growth Neutron Macromolecular Crystallography Crystallization Approaches Dialysis Method Protein Solution Precipitant Solution Semi-permeable Membrane Supersaturation Nucleation OptiCrys Temperature Controlled Dialysis Crystallization Micro Dialysis Buttons Natural Macromolecular Crystallography Crystallization Phase Diagram Lysozyme Solution Dome Shape Air Bubble Formation Applicator Elastic Or Ring Membrane Fixation Crystallization Solution
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