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Visualização bidimensional e quantificação de labile, nutrientes vegetais inorgânicos e contaminantes no solo
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1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry,Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group,University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy,University of Natural Resources and Life Sciences, Vienna
Capítulos
- 00:04Introduction
- 00:51Diffusive Gradients in Thin Films (DGT) Gel Fabrication and Rhizotron Preparation
- 03:06Plant Cultivation
- 03:47Solute Distribution Sampling
- 04:48Gel Retrieval and Chemical Analysis Preparation
- 05:38Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) Line Scan Analysis
- 07:39Data Processing and Calibration
- 09:03Image Generation
- 09:48Results: Representative Sub-mm 2D Solute Flux Distribution of Multiple Nutrients and Contaminants Across Different Soil-Root Interfaces
- 11:24Conclusion
Este protocolo apresenta um fluxo de trabalho para visualização sub-mm 2D de múltiplas espécies de nutrientes inorgânicos labile e súute contaminante usando gradientes difusivos em filmes finos (DGT) combinados com imagens de espectrometria de massa. A amostragem de soluto e a análise química de alta resolução são descritas em detalhes para mapeamento quantitativo de solutos na rizosfera das plantas terrestres.
