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An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
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An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
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09:52 min
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September 15, 2020
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Tracking individual translation events allows for high-resolution kinetic studies of cap-dependent translation mechanisms. Here we demonstrate an in vitro single-molecule assay based on imaging interactions between fluorescently labeled antibodies and epitope-tagged nascent peptides. This method enables single-molecule characterization of initiation and peptide elongation kinetics during active in vitro cap-dependent translation.
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Gaba, A., Wang, H., Qu, X. An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics. J. Vis. Exp. (163), e61648, doi:10.3791/61648 (2020).
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