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Akış Sitometri Analizi için Fare Böbrek-Resident CD8+ T hücrelerinin izolasyonu
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JoVE Revista Inmunología y contagio
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Akış Sitometri Analizi için Fare Böbrek-Resident CD8+ T hücrelerinin izolasyonu

6,125 Views

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06:07 min

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June 27, 2020

DOI:

10.3791/61559-v

DOI:
10.3791/61559-v

•

06:07 min
June 27, 2020

•

11 Views
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1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Summary

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Viral enfeksiyonutaki böbrek, nispeten çok sayıda CD8+ T hücrebarındırır ve mukozal olmayan TRM hücrelerini inceleme fırsatı sunar. Burada, akış sitometri analizi için fare böbrek lenfositleri izole etmek için bir protokol açıklar.

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Cite this Article

Liao, W., Ma, C., Zhang, N. Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J. Vis. Exp. (160), e61559, doi:10.3791/61559 (2020).

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