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Verdauung von Ganzmausaugen für multiparameterflow Cytometric Analysis of Mononuclear Phagozyten
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JoVE Revista Inmunología y contagio
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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Verdauung von Ganzmausaugen für multiparameterflow Cytometric Analysis of Mononuclear Phagozyten

4,697 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

4 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Tags

DigestionMouse EyesMononuclear PhagocytesFlow CytometryMacrophagesMonocytesMicrogliaDendritic CellsCell Surface MarkersChoroidal NeovascularizationDiabetic RetinopathyExperimental Autoimmune UveitisLupusCytometer ConfigurationDissectionMechanical Disruption

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Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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