Journal
/
Inmunología y contagio
/
Optimization of the Cuff Technique for Murine Heart Transplantation
JoVE Revista
Inmunología y contagio
Author Produced
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Optimization of the Cuff Technique for Murine Heart Transplantation

Optimization of the Cuff Technique for Murine Heart Transplantation

DOI:
10.3791/61103-v

•

14:01 min

•

June 26, 2020

•

, , , , , , , ,
1Organ Transplantation Institute, School of Medicine,Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation,The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center,Central South University, 6School of Medicine,Xiamen University, 7Xiamen Medical College, 8Medical College,Guangxi University

Capítulos

  • 00:01Introduction
  • 01:32Recipient Preparation
  • 07:06Donor Preparation
  • 11:35Representative Results
  • 13:39Conclusion

Summary

Traducción Automática

Traducción Automática

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

Tags

Cuff TechniqueCardiac TransplantationSurgical EfficiencyTube InsertionThermal Surgeon Operation ModuleExternal Jugular VeinCarotid ArteryBlunt DissectionMicro ForcepsSuture

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Murine Model of CD40-activation of B cells

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Optimization of a Quantitative Micro-neutralization Assay

Murine Full-thickness Skin Transplantation

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Isolation and Identification of Extravascular Immune Cells of the Heart

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Read Article