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09:27 min
May 06, 2020
DOI:
10.3791/61090-v
这里介绍的是原发性人类血液单细胞分离的协议,以及它们分化成巨噬细胞和树突状细胞,并组装上皮细胞成多细胞人类肺模型。比较了由免疫细胞组成的生物反应,这些细胞与新鲜分离或解冻的单核细胞不同,在接触刺激性刺激时。
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Barosova, H., Drasler, B., Petri-Fink, A., Rothen-Rutishauser, B. Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment. J. Vis. Exp. (159), e61090, doi:10.3791/61090 (2020).
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