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Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture
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Neurociencias
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JoVE Revista Neurociencias
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

DOI:
10.3791/60989-v

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09:40 min

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April 02, 2020

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, , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capítulos

  • 00:04Introduction
  • 00:38Preparation of Dil/Tungsten Bead Tubing
  • 02:55Preparation of Brain Sections
  • 04:20Ballistic Labeling and Visualization of Brain Sections
  • 06:16Cell Culture Method
  • 07:12Results: Analysis of Pyramidal Neurons Using Ballistic Labeling Technology
  • 08:44Conclusion

Summary

Traducción Automática

Traducción Automática

We present a protocol to label and analyze pyramidal neurons, which is critical for evaluating potential morphological alterations in neurons and dendritic spines that may underlie neurochemical and behavioral abnormalities.

Tags

Ballistic LabelingPyramidal NeuronsBrain SlicesPrimary Cell CulturePVP SolutionTungsten Microcarrier BeadsLipophilic DiIC18(3) DyeMethylene ChlorideSonicationNitrogen Gas DryingRat Brain PerfusionPBSPFA

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