Journal
/
Quimica
/
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
JoVE Revista
Quimica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Quimica
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

DOI:
10.3791/60945-v

•

10:45 min

•

June 20, 2020

•

, , , ,
1SUGAR Program, X-star,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group,Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Capítulos

  • 00:04Introduction
  • 00:33Cleaning and Silanizing the Glass
  • 01:26Spin-Coating the Glass Substrate
  • 02:40Photolithography, Etching, and Cleaning
  • 03:47Fabricating the Microchamber Array Device
  • 05:04Generating the Femtoliter Droplet Array (FemDA)
  • 06:35Image Data Analysis
  • 08:45Results: Cell-Free Protein Synthesis in FemDA
  • 10:07Conclusion

Summary

Traducción Automática

Traducción Automática

The overall goal of the protocol is to prepare over one million ordered, uniform, stable, and biocompatible femtoliter droplets on a 1 cm2 planar substrate that can be used for cell-free protein synthesis.

Tags

Keywords: Femtoliter Droplet ArraySingle DNA MoleculesParallel Protein SynthesisEnzyme Activity MeasurementMicrofabricationPhotolithographyCYTOP PolymerSpin CoatingReactive-ion Etching

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/60945?article_type=v

Surface Passivation for Single-molecule Protein Studies
check_url/es/60945?article_type=v

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
check_url/es/60945?article_type=v

Folding and Characterization of a Bio-responsive Robot from DNA Origami
check_url/es/60945?article_type=v

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
check_url/es/60945?article_type=v

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
check_url/es/60945?article_type=v

Synthesis of a Water-soluble Metal–Organic Complex Array
check_url/es/60945?article_type=v

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
check_url/es/60945?article_type=v

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
check_url/es/60945?article_type=v

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction
check_url/es/60945?article_type=v

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Read Article