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无目标液相色谱-质量光谱学-小麦粒基代谢代谢学分析
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07:10 min
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March 13, 2020
DOI:
1Research and Innovation,Murdoch University, 2Centre for Digital Agriculture,Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre,Murdoch University
Summary
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提出了一种对小麦粒代谢物和脂质进行非靶向分析的方法。该协议包括醋酸代谢物提取方法和反向相液色谱-质谱方法，在正负电喷雾电相成像模式下采集。
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Abbiss, H., Gummer, J. P. A., Francki, M., Trengove, R. D. Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain. J. Vis. Exp. (157), e60851, doi:10.3791/60851 (2020).
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