Journal
/
Bioquímica
/
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

5,599 Views

•

10:12 min

•

March 25, 2020

DOI:

10.3791/60734-v

DOI:
10.3791/60734-v

•

10:12 min
March 25, 2020

•

9 Views
, , , , ,
1Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, 2Department of Chemistry,University of Illinois at Chicago

Summary

Automatically generated

Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.

Explore More Videos

Cholesterol EnrichmentXenopus OocytesHypercholesterolemiaCellular MembranesCerebral ArteriesCircle Of WillisMethyl-beta-cyclodextrinCholesterol Saturated SolutionLipid HandlingFilipin Staining

Read Article

Cite this Article

Slayden, A., North, K., Bisen, S., Dopico, A. M., Bukiya, A. N., Rosenhouse-Dantsker, A. Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol. J. Vis. Exp. (157), e60734, doi:10.3791/60734 (2020).

Download .ris file

Copy

Share Video

.

Copy