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Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
JoVE Revista
Medicina
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JoVE Revista Medicina
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

DOI:
10.3791/60714-v

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06:55 min

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February 22, 2020

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, , ,
1Department of Biochemistry and Molecular Genetics,University of Alabama at Birmingham

Capítulos

  • 00:05Introduction
  • 00:52Colorimetric Lactic Dehydrogenase (LDH) Measurement-Based Natural Killer (NK) Cell-Mediated Cytotoxicity Assay
  • 02:46Calcein AM Staining-Based NK Cell-Mediated Cytotoxicity Assay
  • 04:10NK Cell Migration Assay
  • 05:26Results: Representative NK Cell-Mediated Cytoxicity and Migration
  • 06:17Conclusion

Summary

Traducción Automática

Traducción Automática

Evasion of natural killer (NK) cell-mediated eradication by cancer cells is important for cancer initiation and progression. Here, we present two non-radioactivity-based protocols to evaluate NK cell-mediated cytotoxicity toward hepatic tumor cells. Additionally, a third protocol is presented to analyze NK cell migration.

Tags

NK Cell FunctionNK Cell-mediated CytotoxicityNK Cell MigrationTumor MicroenvironmentLDH AssayCalcein AM AssayHuman Liver Cancer CellsCell CultureCentrifugation96-well PlateAbsorbance MeasurementCytotoxicity Calculation

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