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Produção de partículas pseudodigitadas de gripe infecciosa de alto titer com glicoproteínas envelope de vírus H5N1 e H7N9 altamente patogênicos
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JoVE Revista Inmunología y contagio
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Produção de partículas pseudodigitadas de gripe infecciosa de alto titer com glicoproteínas envelope de vírus H5N1 e H7N9 altamente patogênicos

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08:10 min

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January 15, 2020

DOI:

10.3791/60663-v

DOI:
10.3791/60663-v

•

08:10 min
January 15, 2020

•

1 Views
, , , , , ,
1Central Laboratory,Hang Zhou Red Cross Hospital, 2Clinical Laboratory,Yuhang District Maternity and Child Health Care Hospital

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InfluenzaPseudotyped ParticlesViral Envelope GlycoproteinsH5N1H7N9BSL-2TransfectionQRT-PCRVirus ReassortmentCell CultureIncubationHarvestQuantificationBenzonase Nuclease

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Zhang, F., Wang, Y., Shang, X., Wang, S., Xiao, R., Zhou, H., Cai, L. Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses. J. Vis. Exp. (155), e60663, doi:10.3791/60663 (2020).

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