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膜和细胞壁选择性荧光染料在丝状真菌活细胞成像中的应用
JoVE Revista
Biología
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JoVE Revista Biología
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
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膜和细胞壁选择性荧光染料在丝状真菌活细胞成像中的应用

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07:44 min

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November 28, 2019

DOI:

10.3791/60613-v

DOI:
10.3791/60613-v

•

07:44 min
November 28, 2019

•

47 Views
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1Department of Microbiology,University of Innsbruck

Summary

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生命荧光染料是现代真菌细胞生物学中活细胞成像分析的重要工具。本文详细介绍了已建立的和鲜为人知的荧光染料在丝状真菌中跟踪血浆膜动力学、内膜/外细胞化和细胞壁形态发生中的应用。

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Lichius, A., Zeilinger, S. Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi. J. Vis. Exp. (153), e60613, doi:10.3791/60613 (2019).

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