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Plataforma de trasplante de médula ósea para investigar el papel de las células dendríticas en la enfermedad de injerto contra huésped
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
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Plataforma de trasplante de médula ósea para investigar el papel de las células dendríticas en la enfermedad de injerto contra huésped

7,569 Views

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08:05 min

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March 17, 2020

DOI:

10.3791/60083-v

DOI:
10.3791/60083-v

•

08:05 min
March 17, 2020

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8 Views
, , , , , , , ,
1Cancer Division, Burnett School of Biomedical Sciences,University of Central Florida, 2Department of Biochemistry,Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences,Houston Methodist Research Institute, 4Department of Epidemiology,University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences,University of Pittsburgh Medical Center, Hillman Cancer Center

Tags

Bone Marrow TransplantationDendritic CellsGraft-versus-host DiseaseT-cell DepletionCell IsolationCell CultureImmune Cell PopulationsMacrophagesNeutrophilsCell RegenerationBone Marrow PreparationCell HarvestingCell StrainingCell WashingCell ResuspensionCell IncubationCell CountingCell Pooling

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Nguyen, H. D., Huong, P. T., Hossack, K., Gurshaney, S., Ezhakunnel, K., Huynh, T., Alvarez, A. M., Le, N., Luu, H. N. Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease. J. Vis. Exp. (157), e60083, doi:10.3791/60083 (2020).

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