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Real-time, semi-automatisert fluorescerende måling av luftveiene overflaten flytende pH av primær menneskelige Luftveisepitel celler
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Real-time, semi-automatisert fluorescerende måling av luftveiene overflaten flytende pH av primær menneskelige Luftveisepitel celler
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June 13, 2019
DOI:
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
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Real-timeSemi-automatedFluorescent MeasurementAirway Surface Liquid PHPrimary Human Airway Epithelial CellsIon ChannelsTransportersExtracellular PH RegulationCystic FibrosisAsthmaCOPDAir-liquid InterfaceBicarbonate BufferPlate ReaderTemperature ControlCarbon Dioxide ControlHumidity CassetteKinetic Loop
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Saint-Criq, V., Haq, I. J., Gardner, A. I., Garnett, J. P., Ward, C., Brodlie, M., Gray, M. A. Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells. J. Vis. Exp. (148), e59815, doi:10.3791/59815 (2019).
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