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June 08, 2019
DOI:
1Center for Genetic Medicine,Children’s National Health System, 2Institute for Biomedical Sciences,The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology,University of California San Francisco, 5DIPG Centre of Expertise Zurich,Universitats-Kinderspital Zurich
Summary
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在这里,我们提出一个协议,以检测肿瘤体突变在循环DNA存在于患者生物流体(生物流体)。我们的滴数字聚合酶链反应(dPCR)方法能够定量肿瘤突变等位基因频率(MAF),促进对肿瘤反应的诊断和时间监测的微创补充。
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Cite this Article
Bonner, E. R., Saoud, K., Lee, S., Panditharatna, E., Kambhampati, M., Mueller, S., Nazarian, J. Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids. J. Vis. Exp. (148), e59721, doi:10.3791/59721 (2019).
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