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Cromatografia a scambio ionico (IEX) accoppiata alla diffusione della luce multi-angolo (MALS) per la caratterizzazione e la separazione delle proteine
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Bioquímica
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JoVE Revista Bioquímica
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
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Cromatografia a scambio ionico (IEX) accoppiata alla diffusione della luce multi-angolo (MALS) per la caratterizzazione e la separazione delle proteine

16,904 Views

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10:41 min

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April 05, 2019

DOI:

10.3791/59408-v

DOI:
10.3791/59408-v

•

10:41 min
April 05, 2019

•

5 Views
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1Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 2Wyatt Technology Corporation, 3Danyel Biotech Ltd

Tags

Ion Exchange ChromatographyIEXMulti-angle Light ScatteringMALSProtein SeparationProtein CharacterizationProtein Quality ControlOligomeric StateHomogeneityIdentityConformationStructurePost-translation ModificationsAnion ExchangeAIEXCation ExchangeCIEXBSABufferFPLCDLS

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Amartely, H., Some, D., Tsadok, A., Lebendiker, M. Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization. J. Vis. Exp. (146), e59408, doi:10.3791/59408 (2019).

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