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Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
 

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Article DOI: 10.3791/59251-v • 10:09 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 3, 1, 4, 1
1The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 2School of Forestry, Inner Mongolia Agricultural University, 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 4School of Forestry, Fujian Agriculture and Forestry University

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In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.

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Keywords: Sample Preparation Scanning Electron Microscope Transmission Electron Microscope Woodboring Beetle Tween-20 Fixative Fluorescence Microscope Slicing Accuracy Chlorophorus Caragana Appendages Dehydration Carbon Double-sided Adhesive Tape Ion Sputtering Platinum Coating
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