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一种用于阴南芥叶片安装的通用方法

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

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一种用于阴南芥叶片安装的通用方法

7,902 Views

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07:52 min

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February 11, 2019

DOI:

10.3791/59147-v

DOI:

10.3791/59147-v

•

07:52 min

February 11, 2019

•

13 Views

Shigeyuki Betsuyaku^1,2,3,4, Nobuhiko Nomura^3,4, Hiroo Fukuda²

¹Japan Science and Technology Agency (JST), PRESTO, ²Department of Biological Sciences, Graduate School of Science,The University of Tokyo, ³Faculty of Life and Environmental Sciences,University of Tsukuba, ⁴Microbiology Research Center for Sustainability,University of Tsukuba

Summary

我们报告了一种简单和通用的方法, 用于在较长时间内对拟南芥叶片进行荧光实时成像。我们使用转基因拟南芥植物表达荧光记者基因在免疫相关的启动子控制下作为一个例子, 以获得时空了解植物免疫反应。

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