Journal
/
Genética
/
Genetik Mühendisliği hücreleri Dictyostelium discoideum alan seçimi ve bakteri büyüme
JoVE Revista
Genética
This content is Free Access.
JoVE Revista Genética
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Please note that all translations are automatically generated. Click here for the English version.

Genetik Mühendisliği hücreleri Dictyostelium discoideum alan seçimi ve bakteri büyüme

12,346 Views

•

06:08 min

•

January 25, 2019

DOI:

10.3791/58981-v

DOI:
10.3791/58981-v

•

06:08 min
January 25, 2019

•

11 Views
, , , , , , ,
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology,University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology,University College London, 5Department of Cell and Developmental Biology,University College London

Summary

Automatically generated

Dictyostelium discoideum hücre göç, endositoz ve geliştirme gibi karmaşık hücresel süreçler çalışmaya bir popüler bir model organizmadır. Belgili tanımlık yarar organizmanın genetik manipülasyon fizibilite üzerinde bağlıdır. Burada, sıvı ortam kodlamayla hücrelerde varolan sınırlamalar üstesinden Dictyostelium discoideum hücre transfect yöntemleri mevcut.

Explore More Videos

Dictyostelium DiscoideumGenetic EngineeringBacterial SelectionGrowth On BacteriaExochromosomal Plasmid TransfectionMicroscopyKlebsiella AerogenesSorensen BufferLB MediumOptical DensityH40 Electroporation BufferSM MediumAgar PlateCell TransferCentrifugationDNA Transformation

Read Article

Cite this Article

Paschke, P., Knecht, D. A., Williams, T. D., Thomason, P. A., Insall, R. H., Chubb, J. R., Kay, R. R., Veltman, D. M. Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria. J. Vis. Exp. (143), e58981, doi:10.3791/58981 (2019).

Download .ris file

Copy

Share Video

.

Copy