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Identifikation af mus og humant antistof repertoirer af næste generations sekventering
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identifikation af mus og humant antistof repertoirer af næste generations sekventering

DOI:
10.3791/58804-v

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08:51 min

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March 15, 2019

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, , , , , , , , , , , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Center for Influenza Virus Research,National Institute of Infectious Diseases, 3School of Science and Technology,Kwansei Gakuin University, 4Department of Pathology,National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases,Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control,Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control,Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE,Hokkaido University, 9Department of Immunology,National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences,University of Tsukuba

Capítulos

  • 00:04Título
  • 01:23Nucleic Acid Isolation from Immune Cells and Tissues
  • 03:04cDNA Synthesis and PCR Amplification
  • 04:58NGS Sequencing of Libraries
  • 05:33Results: Identification of Mouse and Human Antibody Repertoires
  • 08:11Conclusion

Summary

Traducción Automática

Traducción Automática

Her beskriver vi protokoller for analyse og visualisering af strukturen og forfatning af hele antistof repertoirer. Dette indebærer erhvervelse af enorme sekvenser af antistof RNA ved hjælp af næste generation sequencing.

Tags

Antibody RepertoireNext-generation SequencingB Cell RepertoireImmune ResponseVaccine EfficacyImmunoglobulin AmplificationRNA Extraction

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