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增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis
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增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多

DOI:
10.3791/58751-v

•

12:35 min

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January 04, 2019

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, , , ,
1Anesthesiology Department,The Second Hospital of Dalian Medical University, 2Scientific Research Center,The Second Hospital of Dalian Medical University

Capítulos

  • 00:00Título
  • 01:40Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression
  • 04:28Mouse Peritoneal Macrophage Isolation and Primary Culture
  • 06:57Macrophage Phagocytosis Assay using Flourescence Microscopy
  • 08:28Macrophage Phagocytosis Assay using Flow Cytometer
  • 11:05Resultados
  • 11:45Conclusion

Summary

Traducción Automática

Traducción Automática

在这里, 我们提出了一个评估小鼠腹腔巨噬细胞吞噬使用增强绿色荧光蛋白表达大肠杆菌的方案.

Tags

EGFP-expressing Escherichia ColiPhagocytosisMacrophagesPeritoneal MacrophagesInnate Immune FunctionAgingFluorescence MicroscopyFlow CytometryTA CloningPET SUMO VectorBL21 Competent CellsKanamycin

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