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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
 

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Article DOI: 10.3791/58650-v • 08:41 min • November 8th, 2018
November 8th, 2018 •
1, 2,3, 1, 1,2, 1
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

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This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

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Reproducible DsRNA Microinjection Oviposition Bioassay Mosquitoes House Flies Gene Expression Fecundity Biopesticide Field Quantifiable Doses Multi-generational Lethality Aedes Aegypti Musca Domestica Needle Tip Size Forceps Microscope Slide Chill Table Glass Capillaries Nuclease Free Water
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